Amino Acid Quantitative Analysis

Amino Acid Quantitative Analysis N.Koen Introduction Amino acids give the basic building blocks of forming a protein and play an essential role in the energy metabolism, neurotransmission, and lipid transport. Their quantitative analysis is important for various uses, including disease diagnostics and in elucidating nutritional influences on physiology (Fromm & Hargrove, 2012). Amino acid levels in the body fluids are used to diagnose metabolic deficiencies. Deprived or excessive levels of amino acids can show different defects of deficiencies (Lanpher, 2006). Preparation requirements and sample clean-up make the procedure a slow procedure. While some protocols may provide adequate chromatographic methods and derivatization procedures, that makes it more sufficient and quicker. Leucine is an essential amino acid, which means that it cannot be manufactured in the body. It is also well represented in all the proteins in the body. In vivo leucine kinetics presents a theoretically valid index of protein turnover. C onsequently, isotopically labelled (2H, 3H, 13C, 14C or 15N) leucine is most commonly used for the study of protein metabolism in humans and animals (Fromm & Hargrove, 2012). Literature review Amino acid involvement 1. Amino acid analysis Leucine is an amino acid which is usually obtained by hydrolysis of most common proteins. It was among the first of amino acids to be discovered in 1819 in muscle fibre and wool (Bauman, et al., 1992). Leucine is present in large proportions in haemoglobin. This amino acid is also known for preventing the breakdown of muscle proteins caused by injury or stress (Lanpher, 2006). In addition, Leucine may be beneficial for people suffering from phenylketonuria. Leucine is an essential amino acid, so your body cannot produce it naturally but can only obtain it from food, including protein-rich animal food like fish, chicken, beef, also dairy and eggs. Leucine is classified as a hydrophobic amino acid due to its aliphatic isobutyl side chain. It is encod ed by six codons (UUA, UUG, CUU, CUC, CUA, and CUG) and is a major component of the subunits in ferritin, astacin and other ‘buffer’ proteins. 2. Inborn errors of the metabolism Branched-chain organic acidurias are inborn errors of the metabolism involving the branched-chain amino acids (BCAAs) leucine, isoleucine and valine. These diseases usually involve neurological symptoms. They are treatable with strictly controlled diets and enhancement of detoxification of toxic intermediate metabolites. Detoxification is enhanced through supplementation of glycine, carnitine, biotin and other vitamins where applicable. The most common branched-chain organic acidurias are maple syrup urine disease (MSUD), 3-methylcrotonyl-CoA carboxylase (MCC), propionic aciduria (PCC), methylmalonic aciduria (MMA) and isovaleric aciduria (IVA) deficiency (Heidelberg, 2012). Leucine is involved in a few inborn errors of the metabolism, from which maple syrup urine disease (MSUD) is one of the ma in mentioned. MSUD is caused by a deficiency in the branched-chain alpha-keto acid dehydrogenase complex. (Lanpher, 2006) Ketoacidosis, neurological disorders, and developmental disturbance can all be induced by the accumulations of branched-chain amino acids (BCAAs) and branched-chain alpha-keto-acids (BCKAs) in patients with MSUD. According to clinical investigations on MSUD patients, leucine levels over 400ÃŽ ¼mol/L apparently can cause any clinical problem derived from impaired function of the central nervous system. Damage to neuronal cells found in MSUD patients are presumably because of higher concentrations of both blood BCAAs or BCKAs, especially alpha-keto-isocapronic acids. These clinical data from MSUD patients provide a valuable basis on understanding leucine toxicity in the normal subject. (Fromm & Hargrove, 2012)

SUMMARY Essay Example | Topics and Well Written Essays - 250 words - 2

SUMMARY - Essay Example author then focuses on the support of the legislation and the author figured out that the legislation had not met with any form of criticism and no criticism is very rare. The author then focuses on the incompleteness of the legislation and states that the legislators have not provided advice regarding where the food waste was supposed to be dumped. He cites an individual belonging to the organization of The National Waste and Recycling association who believes that the food waste can be dumped in anaerobic digesters that churn this kind of waste in the same way food is churned in the stomach of a cow. The author states that the food waste can even be dumped at composite sites where this waste along with other waste is transformed into compost and the compost does not smell as bad as raw waste. The author states that companies that are involved in the production of compost will gain some new business due to this legislation and the author even states that similar legislations are gai ning popularity in other states. NPR.org,. Massachusetts Food Waste Ban Gains Broad Acceptance. N.p., 2014. Web. 6 Nov. 2014.

Has Apple done enough to help Foxconn workers Essay

Has Apple done enough to help Foxconn workers - Essay Example 12 on Apple products manufactured in China; there has been mounting criticism of labor malpractices in the manufacturing processes, leading to conduction of investigations by international labor groups into companies which contract to make Apple products. The makers of iPad have specifically largely been criticized for a number of labor related problems, some of which are quite serious in nature (Andrzejcyzk, 2015). Thus the workers who toil night and day at manufacturing plants in China to ensure that Apple customers are satisfied, usually do so under harsh working conditions including work hours beyond the 8 hour day, forced overtime as well as other health and safety risks(Chang, â€Å"Audit Faults Apple Supplier’s Factories in China†).Foxconn Taiwanese company is Apple’s key supplier for the iPad and employs over I million people there. In March 2012,Fair Labor Association(FLA) conducted research(almost 3,000 hours at 3 of the Foxconn factories) and found the following; that the average work week was 60 hours with 36 hours overtime for the month at all the Foxconn manufacturing sites, some employees did not have a single day off for the entire work week, with several employees logging in a 7 day work week before they got a day off, over 60% of employees surveyed claimed that their pay did not cover enough to â€Å"meet basic needs† .A group called Students and Sc holars also compiled a report in May 2012 against Corporate Misbehavior(SACOM).The report derived its content from interviews conducted with factory workers and supervisors in Shenzhen and Zhengzhou, China. The SACOM report cited that while the company increased wages, it also increased production quotas by some 10%.Moreover the living conditions were described as being â€Å"cramped and unpleasant† with at times as many as 30 workers sharing space in an apartment with just 3 bedrooms (Musil, â€Å"Foxconn Working Conditions Slammed by Workers Rights Groups†).Te employees were also strictly warned not